Journal: Journal of Neuroinflammation

Article Title: Astrocytic CCL5 orchestrates CCR5-positive neuronal necroptosis in subarachnoid hemorrhage

doi: 10.1186/s12974-026-03714-5

Figure Lengend Snippet: Astrocytic CCL5 is the primary ligand upregulated after SAH. A . Heatmap of chemokine mRNA expression 3 days post-SAH by qRT-PCR ( n = 3). B . ELISA of CCL5 protein levels in brain tissue at each indicated time ( n = 6). C . Regional CCL5 mRNA expression by qRT-PCR ( n = 5). D - E . Immunofluorescent staining showed that CCL5 was mainly colocalized with GFAP⁺ astrocytes, but not Iba1⁺ microglia, in the hippocampus. Scale bars, 25 μm. Data are mean ± SD; * P < 0.05, ** P < 0.01 vs. sham; one-way ANOVA with Tukey’s test

Article Snippet: Additionally, recombinant CCL5 (rCCL5, 0.5 μg/ mouse; R&D Systems, Minneapolis) was dissolved in PBS and administered to SAH mice via intracerebroventricular injection as previously reported [ ].

Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Staining

Journal: Journal of Neuroinflammation

Article Title: Astrocytic CCL5 orchestrates CCR5-positive neuronal necroptosis in subarachnoid hemorrhage

doi: 10.1186/s12974-026-03714-5

Figure Lengend Snippet: Knockdown of astrocytic CCL5 reduces neuronal death and improves neurobehavioral function. A . Schematic timeline of the experimental protocol. B . Immunofluorescence images showed the expression of CCL5 and GFAP with shCCL5 or vector intervention. Scale bar: 50 μm. C . ELISA analysis of CCL5 levels in the hippocampus with shCCL5 or vector intervention ( n = 6), * P < 0.05 indicated comparison with vector group. D - E . TUNEL staining and quantification of neuronal death in the hippocampus at 3d after SAH ( n = 6), Scale bars, 25 μm. F – H . Western blot and quantification of PSD95 and Syn in the hippocampus at 3d after SAH ( n = 6). I–M. Neurobehavioral tests: Garcia score ( I ), Rotarod test ( J ), Morris water maze escape latency ( K ), distance to platform ( L ), and platform crossings ( M ) ( n = 6). Data are mean ± SD; * P < 0.05 and ** P < 0.01 indicated comparison with sham group. # P < 0.05 indicated comparison with SAH + vector group; one-way ANOVA with Tukey’s test

Article Snippet: Additionally, recombinant CCL5 (rCCL5, 0.5 μg/ mouse; R&D Systems, Minneapolis) was dissolved in PBS and administered to SAH mice via intracerebroventricular injection as previously reported [ ].

Techniques: Knockdown, Immunofluorescence, Expressing, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Comparison, TUNEL Assay, Staining, Western Blot

Journal: Journal of Neuroinflammation

Article Title: Astrocytic CCL5 orchestrates CCR5-positive neuronal necroptosis in subarachnoid hemorrhage

doi: 10.1186/s12974-026-03714-5

Figure Lengend Snippet: CCL5 and CCR5 levels in human CSF correlate with SAH severity and outcome. A . ELISA of CCR5 in CSF from control and SAH patients ( n = 22 control, n = 41 SAH). B . Correlation of CSF level of CCR5 with Hunt-Hess grade of SAH patients. C . CSF levels of CCR5 in patients with good (mRS 0–2) vs. poor (mRS 3–6) outcomes. D . ELISA of CCR5 in CSF from control and SAH patients. E . Correlation of CSF level of CCL5 with Hunt-Hess grade of SAH patients. F . CSF levels of CCL5 in patients with good vs. poor outcomes. Data are mean ± SD; P < 0.05; Student’s t-test or Pearson correlation ( B , E )

Article Snippet: Additionally, recombinant CCL5 (rCCL5, 0.5 μg/ mouse; R&D Systems, Minneapolis) was dissolved in PBS and administered to SAH mice via intracerebroventricular injection as previously reported [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Control